Xenotropic Murine Leukemia Virus-related Virus-associated Chronic Fatigue Syndrome Reveals a Distinct Inflammatory Signature
Published IN VIVO
VINCENT C. LOMBARDI, KATHRYN S. HAGEN, KENNETH W. HUNTER, JOHN W. DIAMOND, JULIE SMITH-GAGEN, WEI YANG and JUDY A. MIKOVITS
Abstract. Background: The recent identification of xenotropic
murine leukemia virus-related virus (XMRV) in the blood of
patients with chronic fatigue syndrome (CFS) establishes that a
retrovirus may play a role in the pathology in this disease.
Knowledge of the immune response might lead to a better
understanding of the role XMRV plays in this syndrome. Our
objective was to investigate the cytokine and chemokine
response in XMRV-associated CFS. Materials and Methods:
Using Luminex multi-analyte profiling technology, we
measured cytokine and chemokine values in the plasma of
XMRV-infected CFS patients and compared these data to those
of healthy controls. Analysis was performed using the Gene
Expression Pattern Analysis Suite and the Random Forest tree
classification algorithm. Results: This study identifies a
signature of 10 cytokines and chemokines which correctly
identifies XMRV/CFS patients with 93% specificity and 96%
sensitivity. Conclusion: These data show, for the first time, an
immunological pattern associated with XMRV/CFS.
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Funny enough, I already made some of those in redlabs and I do not match those upregulations:
ReplyDeleteUp regulated Value Reference range
Il-8 6 0,00 - 15,00 pg/mL
MIP-1(beta) 58 0,00 - 155,00 pg/mL
MIP -1(alpha)
TNF-(alpha) 0 0,00 - 6,00 pg/mL
IL-6 0 0,00 - 5,00 pg/mL
IL-2
IL-12 0 0,00 - 5,00 pg/mL
Eotaxin
MCP-1 115 0,00 - 165,00 pg/mL
Rantes
Do you know if these cytokines and chemokines can vary on a good or a bad day? Because although I tested XMRV- for antibodies at VIP Dx, and not yet tested for cultured XMRV, I do have an altered RNASe-L, Mitochondrial failure, altered CD8, and all the rest of abnormalities observed in CFS. I also started my illness after EBV+CMV+HHV6 infection, so no doubt about my diagnosis. But aparently I do not match the cytokines profile described in this study, which strikes me a bit if that is supposed to be the immunological signature for diagnosing CFS.
The key word there is "associated". It appears they are saying this is a Cytokine Signature for xmrv associated cfs, not just cfs.
ReplyDeleteThe key point to remember is that the cytokine profile from WPI differs from the cytokine profile of the Lights, Bateman and Klimas. Dr. Mikovits has stated this several times. It appears to me that the others found cytokine signatures which indicate an an activated immune system which could be caused by stress; but the WPI's signature shows a th17 bias in the immune system, and no infectious agent other than another retrovirus creates a th17 bias in the immune system. The Lights, Bateman and Klimas are studying a different cohort than WPI, so naturally the cytokine signatures would be different.
ReplyDeleteI find it terribly pretentious to state that who Dr Bateman, Light and Klimas see is not your disease Mrs Carter. As you can read above, Carlitos' cytokine signature does not match Dr Mikovits. We are all in the same boat, whether you want it or not. There are people out there who are "just a little sick" yet they are XMRV positive. This disease does not discriminate, and doctors of this caliber see who wants to see them, because they want to get better.
ReplyDeleteThere is nothing in this paper that links the findings to XMRV. According to Dr. Mikovits' 2009 Invest in ME presentation*, this group of patients is a pure Incline Village outbreak cohort as diagnosed by Dr. Peterson, which means that the immune profile could simply be due to it being a relatively homogeneous cohort and not due to the patients' putative XMRV status. More importantly these samples were collected before the WPI 'discovered' XMRV in CFS patients' blood, therefore XMRV status was not the stratifying factor for patient selection and could still be the result of contamination of samples.
ReplyDelete* http://www.wpinstitute.org/news/docs/Invest_in_ME_20090529_Mikovits.pdf
Could I draw the attention of your British readers to this?
ReplyDeleteInvest in ME have arranged for Dr Judy Mikovits and Annette Whittemore and a number of other researchers/clinicians to meet with British politicians on Thursday 19th May.
IiME rely on supporters to ask our MPs to attend this meeting, which is being held in the Grimond Room at Portcullis House at 10.30am, Thursday 19th May.
http://findyourmp.parliament.uk/
http://www.parliament.uk/about/contacting/mp/
more info at Invest in ME Facebook (you do not need facebook account to view this, it is open to all) http://www.facebook.com/group.php?gid=5804522506#!/group.php?gid=5804522506&v=wall
thank you, and please pass this on
I have had these same tests and mine match the WPI's findings which gives me confidence in the WPI's work.
ReplyDeleteI have ME and I'm XMRV+ by Culture. I do not have CDC Fukuda CFS, although I would meet the criteria.
The point of this study is to emphasise XMRV in people with neuroimmune disease (ME) and not a label of CFS which is a sociopolitical metaphor.
Many people with CFS won't have the same results as they don't have XMRV. (The Lights study and Klimas's 'fatigue' patients, for example).
Why? A label of CFS is diagnosed with chronic fatigue lasting for 6 months minimum of a new pattern/severity and 4 symptoms. It has nothing to do with XMRV or ME.
ME is a neurological disease (ICD:10 G93.3) and defines an inflammatory state. Myalgic Encephalomyelitis.
WPI's work shows this inflammation, which in total coincidence is in a cohort of people infected with an HGRV Retrovirus, an XMRV.
Or maybe not a coincidence.........
John, you are putting forth a straw man argument. Where does the paper attempt to conclude that XMRV causes the cytokine pattern? It doesn't. Maybe you should read the study conclusions again:
ReplyDelete"The results of this study suggest that multiplex cytokine and chemokine analysis in conjunction with XMRV testing may serve as a useful diagnostic for CFS."
AS you said "Anonymous" CFS is a sociopolitical metaphor. This WPI study is a step in the right direction alright. It is vital to identify a set of biomarkers and study that group not a group of CFS diagnosed individuals even if they are Canadian criteria. If use only diagnostic criteria to select patients then you will end up with no meaningful immunological profile.
ReplyDeleteIt is not a straw man argument to state that this paper shows nothing at all about the legitimacy of putative XMRV infection in CFS patients, since the name of the paper is 'Xenotropic Murine Leukemia Virus-related Virus-associated Chronic Fatigue Syndrome Reveals a Distinct Inflammatory Signature'. As I stated in my previous post, the patients in this study were selected from the original Incline Village cohort by Dr. Peterson before the WPI even reported finding XMRV in CFS patients' blood, therefore XMRV was not the stratifying factor in their selection thus the distinct cytokine/chemokine profile reported in the paper could simply be due to it being a relatively homogenous cohort and have nothing at all to do with XMRV.
ReplyDeleteGiven that the WPI reported finding XMRV in >95% of the samples from the original Science paper, with these samples coming from CFS patients located all over the US, it would be interesting to see if the cytokine/chemokine signature reported in this paper also applies to these individuals and not just a pre-selected cohort.
The cytokine profile in Alan Light's studies are exercise induced. You can get a similar but different cytokine profile when "stress" induced too. The point I am making is that the cytokine profile obtained will depend on many factors and is unlikely to be a marker until the cohort is properly filtered and that means matched for age, length of illness, medication, infective agent profile and possibly gender. In addition the genetic profile will alter the cytokine pattern. Phenotypically fibromyalgia sufferers will give a different cytokine signature to those without fibromyalgia.
ReplyDeleteI'll tell you what "CFS" is to me.
ReplyDeleteDr Cheney and Dr Peterson saw a strange "mystery malady" hit north Lake Tahoe in the winter of '84.
All doctors FOUGHT with us about failing to recover, as if this were something we CHOSE, out of some misguided mindset of self destruction.
Only two doctors stepped up to help, so we all went to them. They were swamped by hundreds of desperate patients.
Dr Peterson called the CDC for assistance with this strange, unfamiliar disease, and all they did was join the rest of the medical profession in fighting us.
After a couple of years, the evidence gathered by Dr Cheney and Dr Peterson made it overwhelmingly clear that we weren't lying about what had happened. We WERE chronically sick.
This illness went through a number of different names. The names may have changed, but the illness stayed exactly the same.
In 1987 the CDC convened a meeting to discuss the creation of a definition for was then called the "Raggedy Anne Disease" and other similar clusters of illness across the USA and Canada.
After this meeting, Dr Cheney called me into his office and asked "How would you like to be a prototype for a new syndrome?"
I was surprised at the poor quality of the provisional term the CDC chose, and it must have shown on my face, for Dr Cheney instantly said "It's only temporary while the CDC figures this out. I don't expect the name to last more than a month or two, now that they are finally looking into this"
I promised that I would accurately represent "The Raggedy Anne Disease", now going by the CDC's new name... to the best of my ability.
To me, that "Raggedy Anne Disease", and all the evidence we had at that time, is "CFS".
Thank God for bona fide scientists like Drs. Mikovits and Lombardi!! We will overcome thanks to heros like them.
ReplyDeleteDoes anyone know why there is no audio for any of these 2011 CFSAC videos. I tried them on YouTube with the same result. Other videos, on YouTube, have normal videos.
ReplyDeleteWhat they should have done was to test 3 groups of patients for these signature cytokines and chemokines:
ReplyDeleteThe groups would be:
(1) XMRV-positive with CFS
(2) XMRV-negative with CFS
(3) Healthy control.
Then you could determine whether the observed cytokine signature was due to CFS, or due to XMRV.